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|Section2= |Section7= }} 3,3’,5,5’-Tetramethylbenzidine or TMB is a chromogenic substrate used in staining procedures in immunohistochemistry as well as being a visualising reagent used in enzyme-linked immunosorbent assays (ELISA).〔(Sigma Aldrich Catalog Entry for 3,3′,5,5′-Tetramethylbenzidine )〕 TMB is a white crystal powder that forms a pale blue-green liquid in solution with ethyl acetate. TMB is degraded by sunlight and by fluorescent lights. ==Enzymatic assay== TMB can act as a hydrogen donor for the reduction of hydrogen peroxide to water by peroxidase enzymes such as horseradish peroxidase. The resulting diimine causes the solution to take on a blue colour, and this colour change can be read on a spectrophotometer at a wavelength of 650 nm. The reaction can be halted by addition of acid or another stop reagent. Using sulfuric acid turns TMB yellow. The colour may be read at 450 nm. 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「3,3',5,5'-Tetramethylbenzidine」の詳細全文を読む スポンサード リンク
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