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3-Azidocoumarin : ウィキペディア英語版 | 3-Azidocoumarin
|Section2= }} 3-Azidocoumarin is an organic compound that is used in the area of bioconjugation. It is a derivative of coumarin, a natural product and precursor for the widely used Coumadin. Azidocoumarin has emerged as a widely applicable labeling agent in diverse biological systems. In particular, it participates in the aptly named ''click reaction'' with alkynes. Bioconjugation involves the labeling of certain cellular components and is applicable to fields such a proteomics and functional genomics with a detachable, fluorescent tag.〔Hermanson, G. T. ''Bioconjugate Techniques''; Academic Press: San Diego, 1996.〕 == Synthesis == A common way to produce the 3-azidocoumarin is by condensation of salicylaldehyde and ''N''-acetylglycine or nitroacetate. The intermediate is trapped with sodium azide to produce the 3-azidocoumarin. The isomeric 4-azidocoumarin (CAS# 42373-56-8) product can also be prepared from 4-hydroxycoumarin via the 4-chloro derivative, which reacts with sodium azide.
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