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AmpliPHOX
The ampliPHOX Colorimetric Detection Technology was created as a research tool for the analysis of low-density microarrays by providing rapid, cost effective colorimetric detection with minimal user input. The combination of a simple reagent kit and a small benchtop instrument provide a powerful new detection technology for low density (<2500 spots) microarray applications and is produced by InDevR, Inc. With an investment about ten times lower than that required for a typical microarray scanner, ampliPHOX provides equivalent analytical sensitivity to fluorescence with a simple colorimetric readout.
The key to ampliPHOX Colorimetric Detection Technology is a light-initiated chemical reaction that forms solid polymer spots on the microarray. Biotinylated targets are first captured onto the microarray, and subsequently labeled with a streptavidin-coupled initiator. When a proprietary solution is added and light from the ampliPHOX Reader illuminates the array, polymer grows selectively from only locations where the biotinylated targets were captured. The process occurs in just a few minutes, and can be seen with the unaided eye and subsequently imaged with the ampliPHOX Reader.
==Principles of Operation==
The ampliPHOX assay allows for the detection of biotin-labeled targets using light and a proprietary streptavidin-label (ampliTAG) to initiate the polymerization of a highly optimized monomer solution (ampliPHY). Transparent polymer is formed only on spots where a labeling event has occurred. The contrast of the polymer is enhanced by applying a simple stain (ampliRED) prior to imaging and analysis using the ampliVIEW software. Polymer spots formed are visible to the unaided eye. On the InDevR website, a (video ) is available to illustrate the concept.
Biotin molecules must be incorporated into the microarray target(s) before ampliPHOX detection is performed. This can be achieved using a number of commercially available or custom options, depending on your particular application. Brief descriptions of the key steps and features of the ampliPHOX assay and ampliPHOX Reader are given below.
抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』
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