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|Section2= |Section3= }} Bromobimane (or monobromobimane) is a heterocyclic compound and bimane dye that is used as a reagent in biochemistry. While bromobimane itself is essentially nonfluorescent, it alkylates thiol groups, displacing the bromine and adding the fluorescent tag (λemission = 478 nm) to the thiol. Its alkylating properties are comparable to iodoacetamide. ==Synthesis== Bromobimane is prepared from 3,4-dimethyl-2-pyrazolin-5-one (a condensation product of ethyl 2-methylacetoacetate with hydrazine) by chlorination followed by basic treatment; with aqueous K2CO3 under heterogeneous conditions, the required ''syn''-bimane, 2,3,5,6-tetramethyl-1''H'',7''H''-pyrazolo()pyrazole-1,7-dione, is the major product. It can then be selectively brominated to the target bromobimane (with 1 equivalent of Br2; or dibromobimane, if 2 equivalents of Br2 are used): Bromobimanes are light-sensitive compounds and should be kept refrigerated and protected from light. 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「Bromobimane」の詳細全文を読む スポンサード リンク
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