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CS-BLAST 〔 〕 (Context-Specific BLAST) is a protein sequence search tools that extends BLAST (Basic Local Alignment Search Tool),〔 〕 (Basic Local Alignment Search Tool) by context-specific mutation probabilities. CSI-BLAST (Context-Specific Iterated) BLAST is the context-specific analog of PSI-BLAST 〔 〕 (Position-Specific Iterated) BLAST. CS-BLAST and CSI-BLAST are available as web-server () and can be downloaded freely (). == Method == For aligning two protein sequences, BLAST uses substitution matrix mutation probabilities which only depend on a single amino-acid. The mutation probabilities of an amino-acid, however, are constrained by its structural context: a lysine in an amphipathic alpha helix stabilizes the protein structure and is less likely to mutate than a lysine in a disordered region. CS-BLAST predicts context-specific mutation probabilities that depend on 13 amino acids (the central amino acid and twelve neighboring amino acids). Context-specific mutation probabilities are more accurate than substitution matrix probabilities and improve sequence alignments as well as sequence searching. 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「CS-BLAST」の詳細全文を読む スポンサード リンク
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