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Calycosin
|Section2= }} Calycosin is an O-methylated isoflavone. It can be isolated from ''Astragalus membranaceus'' Bge. var. mongholicus〔(Preparative isolation and purification of calycosin from Astragalus membranaceus Bge. var. mongholicus (Bge.) Hsiao by high-speed counter-current chromatography. Xiaofeng Ma, Tianyou Zhang, Yun Wei, Pengfei Tu, Yingjie Chen and Yoichiro Ito, Journal of Chromatography A, Volume 962, Issues 1-2, 12 July 2002, Pages 243-247 )〕 and ''Trifolium pratense'' L. (red clover).〔(Identification of isoflavones calycosin and pseudobaptigenin in Trifolium pratense. David R. Biggs and Geoffrey A. Lane, Phytochemistry, Volume 17, Issue 9, 1978, Pages 1683-1684 )〕 == Biosynthesis == Isoflavone 3'-hydroxylase uses formononetin, NADPH, H+ and O2 to produce calycosin, NADP+ and H2O.
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