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ColE1
ColE1 is a plasmid found in bacteria. Its name derives from the fact that it carries a gene for colicin E1 (the ''cea'' gene). It also codes for immunity from this product with the ''imm'' gene. In addition, the plasmid has a series of mobility (''mob'') genes. Its size and the presence of a single EcoRI recognition site caused it to be considered as a vector candidate. ==Replication== ColE1 replication begins at the origin. 555bp upstream from this point, RNA polymerase-initiated transcription of RNAII which acts as a pre-primer and begins the synthesis of the leader strand. The transcript folds into a secondary structure which stabilises the interaction between the nascent RNA and the origin's DNA. This hybrid is attacked by RNase H, which cleaves the RNA strand, exposing a 3' hydroxyl group. This allows the extension of the leading strand by DNA Polymerase I. Lagging strand synthesis is later initiated by a primase encoded by the host cell.1
抄文引用元・出典: フリー百科事典『 ウィキペディア(Wikipedia)』 ■ウィキペディアで「ColE1」の詳細全文を読む
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