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| Cotransformation ： ウィキペディア英語版 |
Cotransformation
Cotransformation is the simultaneous transformation of two or more genes.
Only genes in the same chromosomal vicinity can be transformed; the closer together the genes lie, the more frequently they will be cotransformed. By contrast, genes sufficiently far apart that they cannot appear together on a fragment of donor DNA will almost never be cotransformed, because transformation is so inefficient that recipient cells usually take up only a single DNA.
== Example ==
In one study of natural transformation, investigators isolated ''B. subtilis'' bacteria with two mutations—''trpC2'' and ''hisB2''—that made them Trp- , His- auxotrophs. These double auxotrophs served as the recipient in the study, wild-type cells (Trp+ , His+ ) were the donors. In this study, the numbers of Trp+ and His+ transformants were equal. Further tests showed that 40 of every 100 Trp+ transferred colonies were also His+. Similarly, tests of the His+ transformants showed that roughly 40% are also Trp+ . Thus, in 40% of the analyzed colonies, the ''trpC+'' and ''hisB+'' genes had been cotransformed.
抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』
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