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DiI, pronounced like Dye Aye, a chemical with CAS number 41085-99-8, also known as D282 and DiIC18(3), is a fluorescent lipophilic cationic indocarbocyanine dye which is usually made as a perchlorate salt. It is used for scientific staining purposes, such as single molecule imaging, fate mapping, and neuronal tracing (as DiI is retained in the lipid bilayers). DiI is manufactured by Invitrogen, which has a series of long-chain lipophilic carbocyanines, of which DiI is one of the most well researched members. Some prominent members of the series includes: DiI, also called DiIC18(3); DiO, also called DiOC18(3); DiD, also called DiIC18(5); and DiR, also called DiIC18(7), which exhibit distinct orange, green, red and infrared fluorescence, respectively, and all have the following useful properties:〔(Invitrogen Molecular probes Handbook ) 〕〔(detailed data from invitrogen on this series of lipophilic dyes )〕 *Diffuse laterally to stain the entire cell * Fluoresce weakly in water but highly fluorescent and quite photostable when incorporated into membranes * Possess very bright signals with high extinction coefficients * Are well retained in cell membranes * Demonstrate very little transfer to other cells ==Name and Chemical structure== The "Di" possibly stands for the di-alkyl nature and the "I" of DiI possibly stands for the "indocarbocyanine" group (which it shares with D383, D384, D3886, D3899, D3911, D7756, D7776, D7777, D12730, N22880). The full chemical name of this major member of the group is 1,1'-dioctadecyl-3,3,3'3'-tetramethylindocarbocyanine perchlorate, the chemical formula is 抄文引用元・出典: フリー百科事典『 ウィキペディア(Wikipedia)』 ■ウィキペディアで「DiI」の詳細全文を読む スポンサード リンク
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