|
|Section2= |Section3= }} Digoxigenin (DIG) is a steroid found exclusively in the flowers and leaves of the plants ''Digitalis purpurea'', ''Digitalis orientalis'' and ''Digitalis lanata'' (foxgloves), where it is attached to sugars, to form the glycosides (e.g. Lanatoside C). == Use in biotechnology == Digoxigenin is a hapten, a small molecule with high antigenicity (not to be confused with immunogenicity), that is used in many molecular biology applications similarly to other popular haptens such as DNP (dinitrophenol), biotin, and fluorescein. Typically, digoxigenin is introduced chemically (conjugation) into biomolecules (proteins, nucleic acids) to be detected in further assays. Anti-digoxigenin antibodies with high affinities and specificity, are used in a variety of biological immuno-assays (e.g. ELISA). The antibodies are labeled with dyes, enzymes or fluorescence, directly or secondarily, for visualization and detection. Digoxigenin is thus an all-purpose immuno-tag, and in particular a standard immunohistochemical marker for in situ hybridization. In this case it is conjugated to a single species of RNA nucleotide triphosphate (typically uridine), which is then incorporated into RNA (a "riboprobe") as it is synthesized by the cellular machinery. It allows to make : * sensitive non-radioactive in situ hybridization probes to detect nucleic acids in plants, able to detect 1 µg of plasmid DNA. * peptide-DIG conjugates, i.e. bradykinin assay by very sensitive chemiluminescence immunoassays. * fluorescent and DIG-labeled tracers for competitive immunoassays, i.e. to limit detect digoxin, a drug used to cure cardiac arrhythmia, down to 0.2 ng mL−1. * Digoxigenin may be conjugated to sugars to study glycosylation events, even in biological systems. 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「Digoxigenin」の詳細全文を読む スポンサード リンク
|