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FlAsH-EDT2
FlAsH-EDT2 is an organoarsenic compound used in bioanalytical research. It is a fluorescent label. The compound is a derivative of fluorescein. The fluorescin arsenical hairpin binder-ethanedithiol, more commonly known as FlAsH-EDT2, is a pale yellow or pinkish fluorogenic solid with the molecular formula (C2H4AsS2)2-(C20H10O5). It is used in site-specific labeling of living cells. It binds to proteins containing the tetracysteine motif Cys-Cys-Xxx-Xxx-Cys-Cys and becomes fluorescent. It has been seen in studies however that it will also bind non-specifically, meaning simply a site other than the main site of interest (CCXXCC), to endogenous cysteine-rich proteins.
==Preparation==
FlAsH-EDT2 can be prepared in three steps from fluorescein. In the first step, fluorescein is treated with mercury oxide and tetrafluoroacetic acid to produce fluorescein mercuric acetate, a yellow orange solid upon the completion of purification. Treating fluorescein mercuric acetate with arsenic trichloride and palladium acetate under the inert atmosphere goes through the transmetallation to give a yellow solution. To this reaction mixture, addition of 1,2-ethanedithiol functionalize thiarsolanyl to achieve the desired product, FlAsH-EDT2(a pale yellow or pinkish solid).
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