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GFP-cDNA
The GFP-cDNA project documents the localisation of proteins to subcellular compartments of the eukaryotic cell applying fluorescence microscopy. Experimental data are complemented with bioinformatic analyses and published online in a database. A search function allows the finding of proteins containing features or motifs of particular interest. The project is a collaboration of the research groups of (Rainer Pepperkok ) at the European Molecular Biology Laboratory (EMBL) and (Stefan Wiemann ) at the German Cancer Research Centre (DKFZ). ==What kinds of experiments are made?== The cDNAs of novel identified Open Reading Frames(ORF) are tagged with Green Fluorescent Protein (GFP) and expressed in eukaryotic cells. Subsequently, the subcellular localisation of the fusion proteins is recorded by fluorescence microscopy. Steps:
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