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HIS3 The HIS3 gene, found in the ''Saccharomyces cerevisiae'' yeast, encodes a protein called Imidazoleglycerol-phosphate dehydratase which catalyses the sixth step in histidine biosynthesis.〔(HIS3/YOR202W Summary )〕 It is analogous to hisB in ''Escherichia coli''. == Exploits == Mutations in ''Escherichia colis analogous gene, hisB allows researchers to select only those individuals expressing the HIS3 gene included on a plasmid. The HIS3 gene is coupled to a certain promoter which can only be activated by successful binding of the relevant transcription factors. This is used in certain methods of bacterial two-hybrid screening to allow the survival of ''E. coli'' in which a desired protein-DNA or protein-protein interaction is taking place (Joung et al., 2000)
抄文引用元・出典: フリー百科事典『 ウィキペディア(Wikipedia)』 ■ウィキペディアで「HIS3」の詳細全文を読む
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