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Immunofluorescence : ウィキペディア英語版
Immunofluorescence


Immunofluorescence is a technique used for light microscopy with a fluorescence microscope and is used primarily on microbiological samples. This technique uses the specificity of antibodies to their antigen to target fluorescent dyes to specific biomolecule targets within a cell, and therefore allows visualisation of the distribution of the target molecule through the sample. Immunofluorescence is a widely used example of immunostaining and is a specific example of immunohistochemistry that makes use of fluorophores to visualise the location of the antibodies.〔(Immunology/Immunofluorescence Protocol at Protocol-online.org )〕
Immunofluorescence can be used on tissue sections, cultured cell lines, or individual cells, and may be used to analyze the distribution of proteins, glycans, and small biological and non-biological molecules. Immunofluoresence can be used in combination with other, non-antibody methods of fluorescent staining, for example, use of DAPI to label DNA. Several microscope designs can be used for analysis of immunofluorescence samples; the simplest is the epifluorescence microscope, and the confocal microscope is also widely used. Various super-resolution microscope designs that are capable of much higher resolution can also be used.〔(Immunofluorescence Method )〕
==Types of immunofluorescence==

There are two classes of immunofluorescence techniques, primary (or direct) and secondary (or indirect).

抄文引用元・出典: フリー百科事典『 ウィキペディア(Wikipedia)
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