| 翻訳と辞書 |
KinITC
== What is ''kinITC'' ? ==
The method ''kinITC'' for ''kinetic Isothermal Titration Calorimetry'' is an extension of the classical ITC technique in view of obtaining kinetic information in addition to thermodynamic information. It has been described in full in,〔Burnouf D., Ennifar E. ''et al.'', ''kinITC'': a new method for obtaining joint thermodynamic and kinetic data by Isothermal Titration Calorimetry. JACS 134(2012)559-565〕 and in a simplified and less general form in.〔Vander Meulen K.A., Butcher S.E., Characterization of the kinetic and thermodynamic landscape of RNA folding using a novel application of isothermal titration calorimetry. Nucl. Acids Res. 40(2011)2140-51〕 In short, ''kinITC'' allows obtaining with a single technique both the information obtained with classical ITC and with a technique like Surface Plasmon Resonance (SPR). In situations where a chemical reaction proceeds clearly through two successive kinetic steps, the major interest of ''kinITC'' is of being potentially more informative alone〔 than ITC and SPR used jointly, but in a classical way. The experimental limitation for its successful application comes from the response time of the instrument, which prevents from obtaining kinetic information if the reaction proceeds too rapidly. It turns out that this is not so much a severe limitation for many reactions occurring with biological macromolecules. The ''kinITC'' method is thus quite appropriate in biology. Note that, as pionereed long time ago by J. Sturtevant, ITC has already been used for deriving kinetic information in biochemistry, but only in the particular situations of enzyme kinetics.〔Bianconi M.L., Calorimetry of enzyme-catalyzed reactions. Biophysical Chemistry 126(2007)59-64〕
抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』
■ウィキペディアで「KinITC」の詳細全文を読む
スポンサード リンク
| 翻訳と辞書 : 翻訳のためのインターネットリソース |
Copyright(C) kotoba.ne.jp 1997-2016. All Rights Reserved.
|
|