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MassTag-PCR : ウィキペディア英語版
MassTag-PCR
MassTag PCR is a new technology PCR based on Mass Spectrometric detection of end product. This technology is pioneered by scientist from Center for Infection and Immunity (CII) of Mailman School of Public Health at Columbia University, USA.
==Principles==
Like conventional PCR, MassTag-PCR uses primer pairs. The difference is primers used for MassTag PCR are tagged with molecules of known masses or MassCodes. Instead of single pair of primers this technology uses a number of primers, making it a multiplex system. Unlike, conventional Multiplex PCR system, in MassTag PCR more than 15 primer pairs could be used. If DNA from any of the agent of primer panel is present, it will be amplified. Each amplified product will carry its specific Masscodes.
The PCR product is then purified to remove unbound primers, dNTPs, enzyme and other impurities. Finally, the purified PCR products are subject of UV as the chemical bond with nucleic acid and primers are photolabile. As the Masscodes are liberated from PCR products they are detected with a Mass Spectrometer.Presence of specific MassCode indicates the presence of specific pathogen.

抄文引用元・出典: フリー百科事典『 ウィキペディア(Wikipedia)
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