翻訳と辞書 Words near each other ・ Molecular self-assembly ・ Molecular sensor ・ Molecular shuttle ・ Molecular sieve ・ Molecular solid ・ Molecular spacer ・ Molecular Spectra and Molecular Structure IV. Constants of Diatomic Molecules ・ Molecular spring ・ Molecular surface ・ Molecular switch ・ Molecular symmetry ・ Molecular assembler ・ Molecular Autism ・ Molecular autoionization ・ Molecular autopsy ・ Molecular beacon ・ Molecular beam ・ Molecular beam epitaxy ・ Molecular binding ・ Molecular biology ・ Molecular Biology (journal) ・ Molecular Biology and Evolution ・ Molecular Biology Core Facilities ・ Molecular Biology of the Cell ・ Molecular Biology of the Cell (textbook) ・ Molecular biophysics ・ Molecular BioSystems ・ Molecular Biotechnology ・ Molecular Borromean rings ・ Molecular Brain Dictionary Lists mini英和辞書 mini和英辞書 Webster 1913 Latin-English FOLDOC Wikipedia English ウィキペディア

翻訳と辞書　辞書検索 [ 開発暫定版 ] スポンサード リンク Molecular beacon ： ウィキペディア英語版 Molecular beacon Molecular beacons are oligonucleotide hybridization probes that can report the presence of specific nucleic acids in homogenous solutions. The term more often used is molecular beacon probes. Molecular beacons are hairpin shaped molecules with an internally quenched fluorophore whose fluorescence is restored when they bind to a target nucleic acid sequence. This is a novel non-radioactive method for detecting specific sequences of nucleic acids. They are useful in situations where it is either not possible or desirable to isolate the probe-target hybrids from an excess of the hybridization probes. ==Molecular beacon probes== A typical molecular beacon probe is 25 nucleotides long. The middle 15 nucleotides are complementary to the target DNA or RNA and do not base pair with one another, while the five nucleotides at each terminus are complementary to each other rather than to the target DNA. A typical molecular beacon structure can be divided in 4 parts: 1) loop, an 18–30 base pair region of the molecular beacon that is complementary to the target sequence; 2) stem formed by the attachment to both termini of the loop of two short (5 to 7 nucleotide residues) oligonucleotides that are complementary to each other; 3) 5' fluorophore at the 5' end of the molecular beacon, a fluorescent dye is covalently attached; 4) 3' quencher (non fluorescent) dye that is covalently attached to the 3' end of the molecular beacon. When the beacon is in closed loop shape, the quencher resides in proximity to the fluorophore, which results in quenching the fluorescent emission of the latter. If the nucleic acid to be detected is complementary to the strand in the loop, the event of hybridization occurs. The duplex formed between the nucleic acid and the loop is more stable than that of the stem because the former duplex involves more base pairs. This causes the separation of the stem and hence of the fluorophore and the quencher. Once the fluorophore is distantiated from the quencher, illumination of the hybrid with light results in the fluorescent emission. The presence of the emission reports that the event of hybridization has occurred and hence the target nucleic acid sequence is present in the test sample. 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「Molecular beacon」の詳細全文を読む スポンサード リンク 翻訳と辞書 : 翻訳のためのインターネットリソース Copyright(C) kotoba.ne.jp 1997-2016. All Rights Reserved.