|
|Section2= |Section7= }} PIPES is the common name for piperazine-N,N′-bis(2-ethanesulfonic acid), and frequently used buffering agent in biochemistry. It is an ethanesulfonic acid buffer developed by Good et al. in the 1960s. ==Applications== PIPES has pKa (6.76 at 25°C) near the physiological pH which makes it useful in cell culture work. Its effective buffering range is 6.1-7.5 at 25° C. PIPES has been documented minimizing lipid loss when buffering glutaraldehyde histology in plant and animal tissues.〔Salema, R. and Brando, I., J. Submicr. Cytol., 9, 79 (1973).〕〔Schiff, R.I. and Gennaro, J.F., Scaning Electron Microsc., 3, 449 (1979).〕 Fungal zoospore fixation for fluorescence microscopy and electron microscopy were optimized with a combination of glutaraldehyde and formaldehyde in PIPES buffer. It has a negligible capacity to bind divalent ions. 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「PIPES」の詳細全文を読む スポンサード リンク
|