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P''grac'' is an artificial promoter consisting of ''Bacillus subtilis'' ''groE'' promoter, lac operator and ''gsiB'' ribosome binding site. This is a strong IPTG-inducible promoter for ''B. subtilis'', which was first appeared on plasmid pNDH33() and later on pHT01 of the company MoBiTec (). Plasmid pHT01 is supposed to be stable in both ''B. subtilis'' and ''Escherichia coli'', and can be used for expression of proteins in these host strains. Using pNDH33/pHT01 proteins could be produced up to 16% of total protein in ''B. subtilis''. P''grac''100 is an improved version of P''grac'', which can produce up to 30% of total cellular proteins in ''B. subtilis''. == References == * Phan T.P.T., Nguyen H.D. and Schumann W. (2006) ''Protein Expression and Purification'' 46: 189-195. * Nguyen H.D., Phan T.P.T. and Schumann W. (2007) ''Current Microbiology'' 55: 89-93. * Phan T.P.T, Tran L.T., Schumann W. and Nguyen H.D (2015) ''Microbial Cell Factories'' 14: 72. * ''Bacillus subtilis'' Protein Expression System Handbook (2008)(). * Bacillus subtilis Pgrac100 Expression Vectors (2015) () 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「Pgrac」の詳細全文を読む スポンサード リンク
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