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PrimerPlex
PrimerPlex designs oligos for standard multiplex PCR assays and for bead-based suspension array systems such as BioPlex-200 and Luminex 100/200 based on xMAP technology. The assays are used for high-throughput SNP applications such as genotyping, pathogen detection, strain typing, and haplotyping. It is supported on both Windows and Mac. The following oligo design options are supported: # ''Multiplex PCR Primer Design'': Can design highly specific multiplex primers for standard PCR assays for up to 30 sequences. # ''Direct Hybridization Assay (DHA) Oligo Design'': Can design probes for 100 individual targets in a multiplex reaction. The SNPs are located at the center to maximize discrimination. # ''ASPE Assay Oligo Design'': Can design primers for 40 individual targets in a multiplex reaction. The mutation is located at the 3' end. The author company, PREMIER Biosoft, has supported strain differentiation multiplex xMAP assays in one of their other products, AlleleID. ==References==
*(Luminex Corporation ) *(PREMIER Biosoft International Releases version 2.00 of PrimerPlex with Support for Using Proven Pre-designed oligos and Analyzing SNPs of Medium-sized Genomes ) *(A multiplex single nucleotide polymorphism typing assay for detecting mutations that result in decreased fluoroquinolone susceptibility in Salmonella enterica serovars Typhi and Paratyphi A )
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