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R.EcoRII
Restriction endonuclease (REase) EcoRII (pronounced "eco R two") is an enzyme of restriction modification system (RM) naturally found in ''Escherichia coli'', a Gram-negative bacteria. Its molecular mass is 45.2 kDa, being composed of 402 amino acids. ==Mode of action== EcoRII is a bacterial Type IIE〔 (PDF )〕 REase that interacts with two〔(PDF )〕 or (three ) copies of the pseudopalindromic DNA recognition sequence 5'-CCWGG-3' (W = A or T), one being the actual target of cleavage, the other(s) serving as the allosteric activator(s). EcoRII cut target DNA sequence CCWGG generating sticky ends.
抄文引用元・出典: フリー百科事典『 ウィキペディア(Wikipedia)』 ■ウィキペディアで「R.EcoRII」の詳細全文を読む
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