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RL-SAGE Robust LongSage (RL-SAGE) is a version of Serial Analysis of Gene Expression SAGE. It is an improvement over LongSAGE analysis that is less advanced than the latest innovation in SAGE technology, SuperSAGE. == Improvements over previous model ==
RL-SAGE was developed to solve several of the problems inherent in SAGE and LongSAGE protocols, including small insert sizes and low cloning efficiency. Improvements include the ability to generate a library with an insert size of 50 ng mRNA, much smaller than previous LongSAGE insert size of 2 μg mRNA 〔Saha, S., et al. (2002). "Using the transcriptome to annotate the genome." Nat Biotechnol 20(5): 508-512. 〕and using a lower number of ditag polymerase chain reactions (PCR) to obtain a complete cDNA library. 〔Gowda, M., et al. (2004). "Robust-LongSAGE (RL-SAGE): a substantially improved LongSAGE method for gene discovery and transcriptome analysis." Plant Physiol 134(3): 890-897.〕
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