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Selector-technique : ウィキペディア英語版 | Selector-technique
The selector technique allows multiplex amplification of arbitrary sets of genomic sequences. Genomic DNA is digested with restriction enzymes, circularized by hybridisation to selectors and subsequently attached to a vector sequence by ligation. The procedure results in circular DNA molecules with an included general primer pair motif that can be used for amplification by PCR or RCA. ==The selector construct==
A selector consists of two oligonucleotides, one Vector oligonucletide and one Selector probe. Together they form one Selector with target specific ends on each side of a general primer motif.
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