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Spo11 is a protein used in a complex along with Mre11, Rad50 and NBS1 during meiotic recombination. It is also involved in the creation of double stranded breaks in the DNA in the early stages of this process. Its active site contains a tyrosine which ligates and dissociates with DNA to promote break formation. One Spo11 protein is involved per strand of DNA, thus two Spo11 proteins are involved in each double stranded break event. Genetic exchange between two DNA molecules by homologous recombination begins with a break in both strands of DNA—called a double-strand break—and recombination is started by an endonuclease enzyme that cuts the DNA molecule that "receives" the exchanged DNA. In meiosis the enzyme is SPO11, which is related to DNA topoisomerases. Topoisomerases change DNA by transiently breaking one or both strands, passing the unbroken DNA strand or strands through the break and repairing the break; the broken ends of the DNA are covalently linked to topoisomerase. SPO11 is similarly attached to the DNA when it forms double-strand breaks during meiosis. ==Meiotic recombination independent of SPO11== SPO11 is considered to play a predominant role in initiating meiotic recombination. However, recombination may also occur by alternative SPO11-independent mechanisms that can be studied experimentally using ''spo11'' mutants. In the budding yeast ''Sacharomyces cerevisiae'', the meiotic defects in recombination and chromosome disjunction of ''spo11'' mutants are alleviated by X-irradiation. This finding indicates that X-ray induced DNA damages can initiate crossover recombination leading to proper disjunction independently of SPO11. In the worm ''Caenorhabditis elegans'', a homolog of ''spo11'' is ordinarily employed in the initiation of meiotic recombination. However, radiation induced-breaks can also initiate recombination in mutants deleted for this ''spo11'' homolog. Deamination of cytosine resulting in the dU:dG mismatch is one of the most common single-base-altering lesions in non-replicating DNA. ''Spo11'' mutants of the fission yeast ''Schizosaccharomyces pombe'' and ''C. elegans'' undergo meiotic crossover recombination and proper chromosome segregation when dU:dG lesions are produced in their DNA. This crossover recombination does not involve the formation of large numbers of double-strand breaks, but does require uracil DNA-glycosylase, an enzyme that removes uracil from the DNA phosphodiester backbone and initiates base excision repair. Thus, it was proposed that base excision repair of DNA damage such as a uracil base, an abasic site, or a single-strand nick is sufficient to initiate meiotic crossover recombination in ''S. pombe'' and ''C. elegans''.〔 In ''S. pombe'', a mutant defective in the ''spo11'' homolog ''Rec12'' is deficient in meiotic recombination. However recombination can be restored to near normal levels by a deletion in ''rad2'', a gene that encodes an endonuclease involved in Okazaki fragment processing (Farah et al., 2005). Both crossover and non-crossover recombination were increased but double-strand breaks were undetectable. On the basis of the biochemical properties of the rad2 deletion, it was proposed that meiotic recombination can be initiated by DNA lesions other than double-strand breaks, such as nicks and gaps which accumulate during premeiotic DNA replication when Okasaki fragment processing is deficient. The above findings indicate that DNA damages arising from a variety of sources can be repaired by meiotic recombination and that such a process can occur independently of SPO11. 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「Spo11」の詳細全文を読む スポンサード リンク
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