|
TBST (or TTBS) is mixture of Tris-Buffered Saline and Tween 20. It is a buffer used for washing nitrocellulose membrane in western blotting and microtiter plate wells in ELISA assays. ==Contents of TBS-Tween== The following is a sample recipe for TBST: *50 mM Tris *150 mM NaCl *0.05% Tween 20 Adjust pH with HCl to pH 7.6 The simplest way to prepare a TBS-Tween solution is to use TBS-T tablets. They are formulated to give a ready to use TBST solution upon dissolution in 500 ml of deionized water.〔(Medicago AB, (2010) Tris buffered saline with Tween pH 7.6 specification sheet )〕 抄文引用元・出典: フリー百科事典『 ウィキペディア(Wikipedia)』 ■ウィキペディアで「TBST」の詳細全文を読む スポンサード リンク
|