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TNP-ATP is a fluorescent molecule that is able to determine whether or not a protein binds to ATP, and the constants associated with that binding. It is primarily used in fluorescence spectroscopy, but is also very useful as an acceptor molecule in FRET, and as a fluorescent probe in fluorescence microscopy and X-ray crystallography.〔 == Constituent Parts == TNP refers to the chemical compound 2,4,6-trinitrophenol, also known as Picric acid.〔Deng, Xiang, Xiaomei Huang, and Di Wu. "Förster Resonance-energy-transfer Detection of 2,4,6-trinitrophenol Using Copper Nanoclusters." ''Anal'' Bioanal Chem Analytical and Bioanalytical Chemistry'' 407.16 (2015):'' 4607-613. Web.〕 It is a primary constituent of many unexploded landmines, and is a cousin to TNT, but less stable.〔 It is recognized as an environmental contaminant and is toxic to many organisms.〔 It is still commonly used in the manufacture of fireworks, explosives, and rocket fuels, as well as in leather, pharmaceutical, and dye industries.〔 ATP is an essential mediator of life. It is used to overcome unfavorable energy barriers to initiate and fuel chemical reactions.〔 It is also used to drive biological machinery and regulate a number of processes via protein-phosphorylation.〔 However, the proteins that bind ATP for both regulation and enzymatic reactions are very diverse—many yet undiscovered—and for many proteins their relationship to ATP in terms of number of binding sites, binding constants, and dissociation constants remain unclear.〔 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「TNP-ATP」の詳細全文を読む スポンサード リンク
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