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|Section2= |Section3= }} Tabtoxin, also known as wildfire toxin, is a simple monobactam biotoxin produced by ''Pseudomonas syringae''. It is the precursor to the antibiotic tabtoxinine β-lactam.Tabtoxin is a monocyclic β-lactam produced by P. syringae pv. tabaci, coronafaciens, and garcae. Pseudomonas syringae pv. tabaci, the causal agent of the wildfire of tobacco, produces the phytotoxin tabtoxin. tabtoxin-producing bacterium, P. syringae BR2, causes a disease of bean (Phaseolus vulgaris) similar to tobacco wildfire. This organism is closely related to P. syringae pv. tabaci but cannot be classified in the pathovar tabaci because it is not pathogenic on tobacco. Tabtoxin has been shown to be a dipeptide precursor that must undergo hydrolysis by a peptidase to yield the biologically active form, tabtoxinine-p-lactam (TβL). Tabtoxin is required by BR2(R) for both chlorosis and lesion formation on bean. All mutations that affected tabtoxin production, whether spon- taneous deletion or transposon induced, also affected lesion formation, and in all cases, restoration of tabtoxin production also restored pathogenic symptoms. Other factors may be required for BR2 to be pathogenic on bean, but apparently these are in addition to tabtoxin production.〔http://aem.asm.org/content/79/16/5023.long〕〔http://jb.asm.org/content/173/13/4124.long〕 ==PROTEIN== Tabtoxin resistance protein (TTR) is an enzyme that catalyzes the acetylation of tabtoxin rendering tabtoxin-producing pathogens tolerant to their own phytotoxins. According to the structure based detoxification mechanism of TTR, three site-directed mutants Y141F, D130N and Y141F-D130N were constructed and overexpressed in E. coli. The products were then purified and their properties were analyzed by CD and DLS. The crystal structure of TTR complexed with its natural cofactor, acetyl coenzyme A (AcCoA), to 1.55 Å resolution. The binary complex forms a characteristic “V” shape for substrate binding and contains the four motifs conserved in the GCN5-related N-acetyltransferase (GNAT) superfamily, which also includes the histone acetyltransferases (HATs). There are reports that TTR possesses HAT activity and suggest an evolutionary relationship between TTR and other GNAT members. The dipeptide toxin contains tabtoxinine-β-lactam (TβL) linked by a peptide bond to threonine produced, the chlorosis-inducing activity occurs only after hydrolysis of the peptide bond by aminopeptidases of plant or bacterial origin. Cleavage of the peptide bond in tabtoxin releases TβL, the toxic moiety. TβL is located at the N terminus, and Thr is at the C terminus, resulting in TβL-Thr. TβL-Thr is hydrolyzed, and the resulting TβL irreversibly inhibits glutamine synthetase, causing characteristic chlorosis in plants. TβL is spontaneously isomerized to tabtoxinine-δ-lactam (TδL), and TblF did not recognize TδL as a substrate, yielding no TδL-Thr. Some characteristics were also provided by them, but only from the perspective of tabtoxin biosynthesis. βL-Thr is observed, but Thr-TβL, whose sequence is the reverse of that of tabtoxin, is not. The effects of carbon, nitrogen sources and amino acids on growth and tabtoxin production by pv. tabaci, were examined by varying the components of a defined basal medium, which contained the following nutrients per liter: sucrose (10 g), KNO3 (5 g), MgSO(4).7H2O (0.2 g), CaCl(2).2H2O (0.11 g), FeSO(4).7H2O (20 mg), NaH2PO(4).2H2O (0.9 g) and H2PO(4).3HO (1 g). Both growth and quantity of tabtoxin synthesized were significantly affected by carbon source, nitrogen source and amino acid supplements. Sorbitol, xylose and sucrose proved to be the best carbon sources for tabtoxin production. Specific toxin production was very low using glucose as a single carbohydrate source, although bacterial growth was well supported by glucose. Amount and type of nitrogen sources (NH4Cl or KNO3) affected the growth of pv. tabaci and quantities of tabtoxin produced. Nitrate is the best of these two forms of nitrogen for production of tabtoxin. 〔http://www.eurekaselect.com/81043/article〕〔http://aem.asm.org/content/79/16/5023.long〕 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「Tabtoxin」の詳細全文を読む スポンサード リンク
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