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An Endoglycosidase is an enzyme that releases oligosaccharides from glycoproteins or glycolipids. It may also cleave polysaccharide chains between residues that are not the terminal residue, although releasing oligosaccharides from conjugated protein and lipid molecules is more common. It breaks the glycosidic bonds between two sugar monomer in the polymer. It is different from exoglycosidase that it does not do so at the terminal residue. Hence, it is used to release long carbohydrates from conjugated molecules. If an exoglycosidase were used, every monomer in the polymer would have to be removed, one by one from the chain, taking a long time. An endoglycosidase cleaves, giving a polymeric product. PROTEIN-x1-x2-x3-x4-x5-x6-x7-x8-x9-x10-x11-...-xn ==Mechanism Overview== The mechanism is an enzymatic hydrolysis that requires two critical molecules; a proton donor (most likely an acid) and a nucleophile(most likely a base).〔 〕 The Endoglycosidases mechanism has two forms; an acid catalyzed protonation of the glycosidic oxygen yielding stereochemical retention at the anomeric carbon or an acid catalyzed protonation of the glycosidic oxygen with a concomitant attack of a water molecule activated by the base residue yielding a stereochemical inversion.〔 Both mechanisms exhibit the same distance between the proton donor and the glycosidic oxygen, situating the proton donor close enough to the glycosidic oxygen for hydrogen bonding.〔 It is the distance between the nucleophile and the anomeric carbon where the two mechanisms begin to diverge. Because the inversion mechanism must accommodate enough space for the water molecule, the nucleophile is situated further away from the anomeric carbon. In the retention mechanism, this distance is only 5.5 -7 angstroms but increases to 9-10 angstroms in the inversion mechanism. Furthermore, the inversion mechanism was found to proceed through a single displacement mechanism involving an oxocarbenium ion-like transition state. Due to the retention mechanism’s proximity between the two carboxyl groups, it goes through a double displacement mechanism that produces a covalent glycosyl-enzyme intermediate. A exoglycosidase would remove each carbohydrate monomer (x) one by one from the end, starting at xn, whereas and endogylcosidase can cut at any glycosidic bond (-) and may cleave after a signature 'link oligosaccharide' that links certain carbohydrates to certain proteins. 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「endoglycosidase」の詳細全文を読む スポンサード リンク
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