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hemocytometer
The hemocytometer is a device used to count cells. It was originally designed for the counting of blood cells.
The hemocytometer was invented by Louis-Charles Malassez and consists of a thick glass microscope slide with a rectangular indentation that creates a chamber. This chamber is engraved with a laser-etched grid of perpendicular lines. The device is carefully crafted so that the area bounded by the lines is known, and the depth of the chamber is also known. It is therefore possible to count the number of cells or particles in a specific volume of fluid, and thereby calculate the concentration of cells in the fluid overall.
==Principles==
The gridded area of the hemocytometer consists of nine 1 x 1 mm (1 mm2) squares. These are subdivided in 3 directions; 0.25 x 0.25 mm (0.0625 mm2), 0.25 x 0.20 mm (0.05 mm2) and 0.20 x 0.20 mm (0.04 mm2). The centralsquare is further subdivided into 0.05 x 0.05 mm (0.0025 mm2) squares. The raised edges of the hemocytometer hold the coverslip 0.1 mm off the marked grid, giving each square a defined volume (see figure on the right).
抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』
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