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indophenol : ウィキペディア英語版
indophenol

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Indophenol is an organic compound with the formula OC6H4NC6H4OH. It is deep blue dye that is the product of the Berthelot's reaction, a common test for ammonia.〔Patton, Charles J.; Crouch, S. R. "Spectrophotometric and kinetics investigation of the Berthelot reaction for the determination of ammonia" Analytical Chemistry 1977, volume 49, 464-9. 〕 The indophenol group, with various substituents in place of O''H'' and various ring substitutions, is found in many dyes used in hair coloring and textiles.〔Horst Berneth "Azine Dyes" in Ullmann's Encyclopedia of Industrial Chemistry 2002, Wiley-VCH, Weinheim. 〕
==Berthelot test==
In the Berthelot test (1859), a sample suspected of containing ammonia is treated with sodium hypochlorite and phenol. The formation of indophenol is used to determine ammonia and paracetamol by spectrophotometry.〔Tsuboi, T.; Hirano, Y.; Shibata, Y.; Motomizu, S.: Sensitivity Improvement of Ammonia Determination Based on Flow-Injection Indophenol Spectrophotometry with Manganese(II) Ion as a Catalyst and Analysis of Exhaust Gas of Thermal Power Plant, Analytical Sciences, October 2002, Vol. 18, pp. 1141/4〕 Other phenols can be used. Dichlorophenol-indophenol (DCPIP), a form of indophenol, is often used to determine the presence of vitamin C, or ascorbic acid.〔David Emlyn Hughes "Titrimetric determination of ascorbic acid with 2,6-dichlorophenol indophenol in commercial liquid diets" Journal of Pharmaceutical Sciences 1983, Volume 72, pages 126–129. 〕

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