|
A precipitin is an antibody which can precipitate out of a solution upon antigen binding. ==Precipitin reaction== The precipitin reaction provided the first quantitative assay for antibody. The precipitin reaction is based upon the interaction of antigen with antibody leading to the production of antigen-antibody complexes. To produce a precipitin reaction, varying amounts of soluble antigen are added to a fixed amount of serum containing antibody. As the amount of antigen added: * In the zone of antibody excess, each molecule of antigen is bound extensively by antibody and crosslinked to other molecules of antigen. The average size of antibody-antigen complex is small; cross-linking between antigen molecules by antibody is rare. * In the zone of equivalence, the formation of precipitin complexes is optimal. Extensive lattices of antigen and antibody are formed by cross-linking. * At high concentrations of antigen, the average size of antibody-antigen complexes is once again small because few antibody molecules are available to cross-link antigen molecules together. The small, soluble immune complexes formed ''in vivo'' in the zone of antigen excess can cause a variety of pathological syndromes. Antibody can only precipitate antigenic substrates that are multivalent—that is, only antigens that have multiple antibody-binding sites epitopes. This allows for the formation of large antigen:antibody complexes. 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「precipitin」の詳細全文を読む スポンサード リンク
|