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|Section2= |Section3= }} Resazurin (7-Hydroxy-3''H''-phenoxazin-3-one 10-oxide) is a blue dye, itself weakly fluorescent until it is irreversibly reduced to the pink colored and highly red fluorescent resorufin. It is used as an oxidation-reduction indicator in cell viability assays for bacteria and mammalian cells, and for measuring aerobic respiration and exchange with the hyporheic zone in streams. Usually it is available commercially as the sodium salt. Resazurin solution has one of the highest values known of Kreft's dichromaticity index. This means that it has a large change in perceived color hue when the thickness or concentration of observed sample increases or decreases. ==Cell Viability applications== Resazurin was first used to quantify bacterial content in milk by Pesch and Simmert in 1929. It is also used as an indicator for cell viability in mammalian cell cultures. It was introduced commercially initially under Alamar Blue trademark (Trek Diagnostic Systems, Inc), and now also available under other names such as AB assay, Vybrant (Molecular Probes) and UptiBlue (Interchim). Resazurin based assays show excellent correlation to reference viability assays such as formazan-based assays (MTT/XTT) and tritiated thymidine based techniques, while being much easier and safer to use for the user.〔(UptiBlue viable cell assay ) technical manual〕 It also allows for longer studies (minimally toxic to living cells), works for adherents cells and bacteria/fungi.〔 Besides standard applications as cell culture assays (cell counting, cell proliferation assays) and cytotoxicity testing, it also can be multiplexed with several chemiluminescent assays, such as cytokine assays, caspase assays to measure apoptosis, or reporter assays to measure a gene or a protein expression.〔 The irreversible reaction of resazurin to resorufin is proportional to aerobic respiration. 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「resazurin」の詳細全文を読む スポンサード リンク
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